VDAC2 sirna

VDAC2 siRNA (Mouse)

Cat. Num. AAA8235916

• Gene Names: Vdac2; Vdac6; mVDAC2; mVDAC6
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VDAC2; VDAC2 siRNA (Mouse); VDAC6; Voltage-dependent anion-selective channel protein 2; VDAC-2; mVDAC2; Outer mitochondrial membrane protein porin 2; Voltage-dependent anion-selective channel protein 6; VDAC-6; mVDAC6; VDAC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: VDAC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 295

• Applicable Applications for VDAC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse VDAC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VDAC2 sirna

siRNA to inhibit VDAC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755965
• NCBI GeneID: 22334
• NCBI Accession #: NP_035825.1
• NCBI GenBank Nucleotide #: NM_011695.2
• UniProt Accession #: Q60930; Q78MH6; Q99L98
• Molecular Weight: 31,733 Da
• NCBI Official Full Name: voltage-dependent anion-selective channel protein 2
• NCBI Official Synonym Full Names: voltage-dependent anion channel 2
• NCBI Official Symbol: Vdac2
• NCBI Official Synonym Symbols: Vdac6; mVDAC2; mVDAC6
• NCBI Protein Information: voltage-dependent anion-selective channel protein 2
• UniProt Protein Name: Voltage-dependent anion-selective channel protein 2
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: Vdac2
• UniProt Synonym Gene Names: Vdac6; VDAC-2; mVDAC2; VDAC-6; mVDAC6
• UniProt Entry Name: VDAC2_MOUSE

Uniprot Description

VDAC2: Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation- selective. Interacts with hexokinases. Expressed in all tissues examined. Belongs to the eukaryotic mitochondrial porin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Mitochondrial; Channel, misc.

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial inner membrane; integral to membrane; nucleus; myelin sheath

Molecular Function: protein binding; nucleotide binding; voltage-gated anion channel activity; porin activity

Biological Process: transport; ion transport; negative regulation of protein polymerization; transmembrane transport; anion transport