USP16 sirna

USP16 siRNA (Mouse)

Cat. Num. AAA8213431

• Gene Names: Usp16; UBPM; 1200004E02Rik; 2810483I07Rik; 6330514E22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP16; USP16 siRNA (Mouse); Ubiquitin carboxyl-terminal hydrolase 16; Deubiquitinating enzyme 16; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16; USP16 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USP16 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 825

• Applicable Applications for USP16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP16 sirna

siRNA to inhibit USP16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 251823802
• NCBI GeneID: 74112
• NCBI Accession #: NP_077220.2
• NCBI GenBank Nucleotide #: NM_024258.2
• UniProt Accession #: Q99LG0; Q99KM0; G5E860
• Molecular Weight: 93,434 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 16
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 16
• NCBI Official Symbol: Usp16
• NCBI Official Synonym Symbols: UBPM; 1200004E02Rik; 2810483I07Rik; 6330514E22Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 16
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 16
• UniProt Gene Name: Usp16
• UniProt Entry Name: UBP16_MOUSE

Uniprot Description

USP16: a ubiquitous ubiquitin-processing protease that may de-ubiquitinate one or more critical proteins that are involved in the condensation of mitotic chromosomes, possibly acting selectively on histones H2A and H2B, the major ubiquitinated proteins of chromatin. Is phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition.

Protein type: Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.12

Cellular Component: nucleus

Molecular Function: peptidase activity; ubiquitin binding; protein binding; hydrolase activity; zinc ion binding; histone binding; metal ion binding; cysteine-type endopeptidase activity; transcription coactivator activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; mitosis; proteasomal ubiquitin-dependent protein catabolic process; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of translational elongation; chromatin modification; proteolysis; cell cycle; histone deubiquitination; protein homotetramerization; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; cell division; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus