USF1 sirna

USF1 siRNA (Mouse)

Cat. Num. AAA8217704

• Gene Names: Usf1; bHLHb11
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USF1; USF1 siRNA (Mouse); USF; Upstream stimulatory factor 1; Major late transcription factor 1; USF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 310

• Applicable Applications for USF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USF1 sirna

siRNA to inhibit USF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 793423971
• NCBI GeneID: 22278
• NCBI Accession #: NP_001292605.1
• NCBI GenBank Nucleotide #: NM_001305676.1
• UniProt Accession #: Q61069
• Molecular Weight: 33,570 Da
• NCBI Official Full Name: upstream stimulatory factor 1 isoform 1
• NCBI Official Synonym Full Names: upstream transcription factor 1
• NCBI Official Symbol: Usf1
• NCBI Official Synonym Symbols: bHLHb11
• NCBI Protein Information: upstream stimulatory factor 1
• UniProt Protein Name: Upstream stimulatory factor 1
• Protein Family: Upstream stimulatory factor
• UniProt Gene Name: Usf1
• UniProt Synonym Gene Names: Usf
• UniProt Entry Name: USF1_MOUSE

NCBI Description

This protein encoded by this gene is a member of the basic-Helix-Hoop-Helix-Leucine zipper (bHLH-LZ) family and encodes a protein that can act as a transcription factor. Studies indicate that the basic region interacts with DNA at E-Box motifs, while the helix-loop-helix and leucine zipper domains are involved in dimerization with different partners. This protein is involved in a wide array of biological pathways, including cell cycle regulation, immune response, and responses to ultraviolet radiation. Mice lacking most of the coding exons of this gene often lacked both whiskers and nasal fur, and were prone to epileptic seizures, while mice lacking both this gene and another family member, Usf2, displayed embryonic lethality (PMID:9520440). Mutations in the human ortholog of this gene have been associated with Familial Combined Hyperlipidemia (FCHL) in humans. Pseudogenes of this gene are found on chromosome 11 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; enzyme binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cellular response to insulin stimulus; positive regulation of transcription, DNA-dependent; response to hypoxia; lipid homeostasis; late viral mRNA transcription; glucose metabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by glucose; response to UV