UCHL5 sirna

UCHL5 siRNA (Mouse)

Cat. Num. AAA8226044

• Gene Names: Uchl5; Uch37; 5830413B11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCHL5; UCHL5 siRNA (Mouse); UCH37; Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH-L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5; UCHL5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UCHL5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 328

• Applicable Applications for UCHL5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UCHL5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCHL5 sirna

siRNA to inhibit UCHL5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 229577283
• NCBI GeneID: 56207
• NCBI Accession #: NP_001153338.1
• NCBI GenBank Nucleotide #: NM_001159866.1
• UniProt Accession #: Q9WUP7; Q9CVJ4; Q9CXZ3; Q9R107
• Molecular Weight: 37,488 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 2
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L5
• NCBI Official Symbol: Uchl5
• NCBI Official Synonym Symbols: Uch37; 5830413B11Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L5
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L5
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl5
• UniProt Synonym Gene Names: Uch37; UCH-L5
• UniProt Entry Name: UCHL5_MOUSE

Uniprot Description

UCHL5: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Belongs to the peptidase C12 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating system; Protease

Cellular Component: proteasome complex; cytoplasm; intracellular; nucleus; cytosol

Molecular Function: peptidase activity; omega peptidase activity; hydrolase activity; ubiquitin-specific protease activity; endopeptidase inhibitor activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; midbrain development; proteolysis; DNA repair; forebrain morphogenesis; response to DNA damage stimulus; lateral ventricle development; DNA recombination