UBA6 sirna

UBA6 siRNA (Mouse)

Cat. Num. AAA8210088

• Gene Names: Uba6; E1-L2; Ube1l2; AU021846; AW124799; 4930542H01; 5730469D23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBA6; UBA6 siRNA (Mouse); UBE1L2; Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Ubiquitin-activating enzyme E1-like protein 2; E1-L2; UBA6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBA6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1053

• Applicable Applications for UBA6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBA6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBA6 sirna

siRNA to inhibit UBA6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27370032
• NCBI GeneID: 231380
• NCBI Accession #: NP_766300.1
• NCBI GenBank Nucleotide #: NM_172712.2
• UniProt Accession #: Q8C7R4
• Molecular Weight: 117,966 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 6
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 6
• NCBI Official Symbol: Uba6
• NCBI Official Synonym Symbols: E1-L2; Ube1l2; AU021846; AW124799; 4930542H01; 5730469D23Rik
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 6
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 6
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: Uba6
• UniProt Synonym Gene Names: Ube1l2; Ubiquitin-activating enzyme 6; E1-L2
• UniProt Entry Name: UBA6_MOUSE

Uniprot Description

UBE1L2: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. Belongs to the ubiquitin-activating E1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; small protein activating enzyme activity; FAT10 activating enzyme activity; nucleotide binding; ubiquitin activating enzyme activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; hippocampus development; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; protein modification process; locomotory behavior; amygdala development; learning