TRPV4 sirna

TRPV4 siRNA (Mouse)

Cat. Num. AAA8200585

• Gene Names: Trpv4; Trp12; VRL-2; VROAC; OTRPC4; VR-OAC; 0610033B08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPV4; TRPV4 siRNA (Mouse); TRP12; VRL2; VROAC; Transient receptor potential cation channel subfamily V member 4; TrpV4; Osm-9-like TRP channel 4; OTRPC4; Transient receptor potential protein 12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR-OAC; TRPV4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TRPV4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 871

• Applicable Applications for TRPV4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TRPV4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPV4 sirna

siRNA to inhibit TRPV4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 269784723
• NCBI GeneID: 63873
• NCBI Accession #: NP_071300.2
• NCBI GenBank Nucleotide #: NM_022017.3
• UniProt Accession #: Q9EPK8; Q91XR5; Q9EQZ4; Q9ERZ7; Q9ES76; A0JNY0
• Molecular Weight: 98,027 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 4
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily V, member 4
• NCBI Official Symbol: Trpv4
• NCBI Official Synonym Symbols: Trp12; VRL-2; VROAC; OTRPC4; VR-OAC; 0610033B08Rik
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpv4
• UniProt Synonym Gene Names: Trp12; Vrl2; Vroac; TrpV4; OTRPC4; TRP12; VR-OAC
• UniProt Entry Name: TRPV4_MOUSE

Uniprot Description

TRPV4: a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: cortical actin cytoskeleton; cell surface; focal adhesion; cell; integral to membrane; cytosol; cilium; growth cone; adherens junction; membrane; cytoplasmic microtubule; lamellipodium; plasma membrane; cytoplasmic vesicle; cell junction; filopodium

Molecular Function: microtubule binding; calcium channel activity; ion channel activity; beta-tubulin binding; nucleotide binding; actin binding; protein kinase binding; calmodulin binding; actin filament binding; protein binding; protein kinase C binding; osmosensor activity; cation channel activity; SH2 domain binding; alpha-tubulin binding; ATP binding

Biological Process: intercellular junction assembly; hypotonic response; actin filament organization; response to osmotic stress; regulation of response to osmotic stress; osmosensory signaling pathway; cellular calcium ion homeostasis; vasopressin secretion; positive regulation of microtubule depolymerization; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; transport; calcium ion transport; actin cytoskeleton reorganization; ion transport; transmembrane transport; cortical microtubule organization and biogenesis; microtubule polymerization