TRABD2B sirna

TRABD2B siRNA (Mouse)

Cat. Num. AAA8201133

• Gene Names: Trabd2b; Hkat; Gm12824; OTTMUSG00000008561
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRABD2B; TRABD2B siRNA (Mouse); GM12824; HKAT; TIKI2; Metalloprotease TIKI2; Heart. kidney and adipose-enriched transmembrane protein; TRAB domain-containing protein 2B; TRABD2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TRABD2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 517

• Applicable Applications for TRABD2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TRABD2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRABD2B sirna

siRNA to inhibit TRABD2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148234945
• NCBI GeneID: 666048
• NCBI Accession #: NP_001079018.1
• NCBI GenBank Nucleotide #: NM_001085549.1
• UniProt Accession #: B1ATG9
• Molecular Weight: 57,368 Da
• NCBI Official Full Name: metalloprotease TIKI2
• NCBI Official Synonym Full Names: TraB domain containing 2B
• NCBI Official Symbol: Trabd2b
• NCBI Official Synonym Symbols: Hkat; Gm12824; OTTMUSG00000008561
• NCBI Protein Information: metalloprotease TIKI2
• UniProt Protein Name: Metalloprotease TIKI2
• Protein Family: Metalloprotease
• UniProt Gene Name: Trabd2b
• UniProt Synonym Gene Names: Gm12824; Hkat; Tiki2
• UniProt Entry Name: TIKI2_MOUSE

Uniprot Description

LOC388630: Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N- terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation. Belongs to the TIKI family.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: peptidase activity; Wnt-protein binding; metallopeptidase activity; hydrolase activity; metal ion binding; metalloendopeptidase activity; cysteine-type peptidase activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; protein amino acid oxidation; metabolic process; proteolysis