TMEM216 sirna

TMEM216 siRNA (Mouse)

Cat. Num. AAA8200272

• Gene Names: Tmem216; AI482550; 1110017C22Rik; 2810441K11Rik; 4921533J23Rik; A930021F15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEM216; TMEM216 siRNA (Mouse); Transmembrane protein 216; Thymus atrophy-related protein; TMEM216 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TMEM216 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 141

• Applicable Applications for TMEM216 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TMEM216 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEM216 sirna

siRNA to inhibit TMEM216 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 482661623
• NCBI GeneID: 68642
• NCBI Accession #: NP_001264789.1
• NCBI GenBank Nucleotide #: NM_001277860.1
• UniProt Accession #: Q9CQC4; D3YUA1
• Molecular Weight: 9,865 Da
• NCBI Official Full Name: transmembrane protein 216 isoform 1
• NCBI Official Synonym Full Names: transmembrane protein 216
• NCBI Official Symbol: Tmem216
• NCBI Official Synonym Symbols: AI482550; 1110017C22Rik; 2810441K11Rik; 4921533J23Rik; A930021F15Rik
• NCBI Protein Information: transmembrane protein 216
• UniProt Protein Name: Transmembrane protein 216
• Protein Family: Transmembrane protein
• UniProt Gene Name: Tmem216
• UniProt Entry Name: TM216_MOUSE

NCBI Description

This gene encodes a transmembrane protein which is involved in regulation of signaling and trafficking of associated proteins. In humans, mutations in this gene are associated with ciliopathies including Joubert, Meckel and related syndromes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]

Uniprot Description

TMEM216: Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition. Defects in TMEM216 are a cause of Joubert syndrome type 2 (JBTS2). JBTS2 is a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Defects in TMEM216 are the cause of Meckel syndrome type 2 (MKS2). It is a form of Meckel syndrome, an autosomal recessive disorder. It is characterized by a combination of renal cysts and variably associated features including developmental anomalies of the central nervous system (typically encephalocele), hepatic ductal dysplasia and cysts, and polydactyly. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cell projection; cytoskeleton; membrane; cytoplasm; integral to membrane; cilium

Biological Process: cell projection organization and biogenesis; cilium biogenesis