TMEM165 sirna

TMEM165 siRNA (Mouse)

Cat. Num. AAA8239484

• Gene Names: Tmem165; Tparl; pFT27; Tpardl; AV026557
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEM165; TMEM165 siRNA (Mouse); TPARL; Transmembrane protein 165; TPA-regulated locus protein; Transmembrane protein PFT27; Transmembrane protein TPARL; TMEM165 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TMEM165 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for TMEM165 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TMEM165 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEM165 sirna

siRNA to inhibit TMEM165 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 111154067
• NCBI GeneID: 21982
• NCBI Accession #: NP_035756.2
• NCBI GenBank Nucleotide #: NM_011626.2
• UniProt Accession #: P52875; Q9R292
• Molecular Weight: 34,791 Da
• NCBI Official Full Name: transmembrane protein 165
• NCBI Official Synonym Full Names: transmembrane protein 165
• NCBI Official Symbol: Tmem165
• NCBI Official Synonym Symbols: Tparl; pFT27; Tpardl; AV026557
• NCBI Protein Information: transmembrane protein 165
• UniProt Protein Name: Transmembrane protein 165
• Protein Family: Transmembrane protein
• UniProt Gene Name: Tmem165
• UniProt Synonym Gene Names: Tparl
• UniProt Entry Name: TM165_MOUSE

Uniprot Description

TMEM165: Defects in TMEM165 are the cause of congenital disorder of glycosylation type 2K (CDG2K). An autosomal recessive disorder with a variable phenotype. Affected individuals show psychomotor retardation and growth retardation, and most have short stature. Other features include dysmorphism, hypotonia, eye abnormalities, acquired microcephaly, hepatomegaly, and skeletal dysplasia. Congenital disorders of glycosylation are caused by a defect in glycoprotein biosynthesis and characterized by under- glycosylated serum glycoproteins and a wide variety of clinical features. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the GDT1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; intracellular membrane-bound organelle; lysosomal membrane; lysosome; integral to membrane; endosome membrane; trans-Golgi network membrane; endosome

Biological Process: cellular calcium ion homeostasis; protein amino acid N-linked glycosylation; Golgi calcium ion transport