TGIF1 sirna

TGIF1 siRNA (Mouse)

Cat. Num. AAA8205607

• Gene Names: Tgif1; Tgif; AA959811; AI462167
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TGIF1; TGIF1 siRNA (Mouse); TGIF; Homeobox protein TGIF1; 5'-TG-3'-interacting factor 1; TGIF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TGIF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 252

• Applicable Applications for TGIF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TGIF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TGIF1 sirna

siRNA to inhibit TGIF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255759999
• NCBI GeneID: 21815
• NCBI Accession #: NP_001157546.1
• NCBI GenBank Nucleotide #: NM_001164074.1
• UniProt Accession #: P70284; Q921F9; Q99JS3
• Molecular Weight: 27,550 Da
• NCBI Official Full Name: homeobox protein TGIF1 isoform c
• NCBI Official Synonym Full Names: TGFB-induced factor homeobox 1
• NCBI Official Symbol: Tgif1
• NCBI Official Synonym Symbols: Tgif; AA959811; AI462167
• NCBI Protein Information: homeobox protein TGIF1
• UniProt Protein Name: Homeobox protein TGIF1
• Protein Family: Homeobox protein
• UniProt Gene Name: Tgif1
• UniProt Synonym Gene Names: Tgif
• UniProt Entry Name: TGIF1_MOUSE

Uniprot Description

TGIF: Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. Defects in TGIF1 are the cause of holoprosencephaly type 4 (HPE4). Holoprosencephaly (HPE) is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Belongs to the TALE/TGIF homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; nucleus

Molecular Function: JUN kinase binding; protein binding; DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; dorsal/ventral pattern formation; retina development in camera-type eye; regulation of gastrulation; neural tube closure; negative regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; positive regulation of neuron differentiation