TFAP2B sirna

TFAP2B siRNA (Mouse)

Cat. Num. AAA8234954

• Gene Names: Tfap2b; Tcfap2b; AI606113; AP-2(beta); E130018K07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TFAP2B; TFAP2B siRNA (Mouse); TCFAP2B; Transcription factor AP-2-beta; AP2-beta; Activating enhancer-binding protein 2-beta; TFAP2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TFAP2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 459

• Applicable Applications for TFAP2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TFAP2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TFAP2B sirna

siRNA to inhibit TFAP2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70609881
• NCBI GeneID: 21419
• NCBI Accession #: NP_033360.2
• NCBI GenBank Nucleotide #: NM_009334.3
• UniProt Accession #: Q61313; Q8CEP1
• Molecular Weight: 50,373 Da
• NCBI Official Full Name: transcription factor AP-2-beta isoform 1
• NCBI Official Synonym Full Names: transcription factor AP-2 beta
• NCBI Official Symbol: Tfap2b
• NCBI Official Synonym Symbols: Tcfap2b; AI606113; AP-2(beta); E130018K07Rik
• NCBI Protein Information: transcription factor AP-2-beta
• UniProt Protein Name: Transcription factor AP-2-beta
• Protein Family: Transcription factor
• UniProt Gene Name: Tfap2b
• UniProt Synonym Gene Names: Tcfap2b; AP2-beta
• UniProt Entry Name: AP2B_MOUSE

Uniprot Description

AP-2 beta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Belongs to the AP-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: cell; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; caspase inhibitor activity; transcription coactivator activity; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; hindlimb morphogenesis; apoptosis; positive regulation of transcription, DNA-dependent; regulation of BMP signaling pathway; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; forelimb morphogenesis; calcium ion homeostasis; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; regulation of cell differentiation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; sodium ion homeostasis; phosphate ion homeostasis; negative regulation of neuron apoptosis; kidney development; potassium ion homeostasis; transcription, DNA-dependent; glucose metabolic process; renal water homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; negative regulation of apoptosis