TECTA sirna

TECTA siRNA (Mouse)

Cat. Num. AAA8221009

• Gene Names: Tecta; Tctna
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TECTA; TECTA siRNA (Mouse); Alpha-tectorin; TECTA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TECTA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,150

• Applicable Applications for TECTA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TECTA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TECTA sirna

siRNA to inhibit TECTA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239787867
• NCBI GeneID: 21683
• NCBI Accession #: NP_033373.2
• NCBI GenBank Nucleotide #: NM_009347.2
• UniProt Accession #: O08523; E9QNR3
• Molecular Weight: 238,898 Da
• NCBI Official Full Name: alpha-tectorin
• NCBI Official Synonym Full Names: tectorin alpha
• NCBI Official Symbol: Tecta
• NCBI Official Synonym Symbols: Tctna
• NCBI Protein Information: alpha-tectorin
• UniProt Protein Name: Alpha-tectorin
• Protein Family: Alpha-tectorin
• UniProt Gene Name: Tecta
• UniProt Entry Name: TECTA_MOUSE

Uniprot Description

TECTA: One of the major non-collagenous components of the tectorial membrane. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals. Defects in TECTA are the cause of deafness autosomal dominant type 12 (DFNA12); also known as DFNA8. DFNA12 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in TECTA are the cause of deafness autosomal recessive type 21 (DFNB21).

Protein type: Extracellular matrix; Membrane protein, GPI anchor

Cellular Component: proteinaceous extracellular matrix; membrane; extracellular region; plasma membrane

Molecular Function: protein binding; extracellular matrix structural constituent

Biological Process: sensory perception of sound; cell-matrix adhesion