TBP sirna

TBP siRNA (Mouse)

Cat. Num. AAA8217011

• Gene Names: Tbp; Gtf2d; SCA17; TFIID; GTF2D1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBP; TBP siRNA (Mouse); TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; TBP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TBP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 316

• Applicable Applications for TBP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBP sirna

siRNA to inhibit TBP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 172073171
• NCBI GeneID: 21374
• NCBI Accession #: NP_038712.3
• NCBI GenBank Nucleotide #: NM_013684.3
• UniProt Accession #: P29037; O09169; Q80UL8; Q8C3S1
• Molecular Weight: 34,709 Da
• NCBI Official Full Name: TATA-box-binding protein
• NCBI Official Synonym Full Names: TATA box binding protein
• NCBI Official Symbol: Tbp
• NCBI Official Synonym Symbols: Gtf2d; SCA17; TFIID; GTF2D1
• NCBI Protein Information: TATA-box-binding protein
• UniProt Protein Name: TATA-box-binding protein
• Protein Family: TBP-like protein
• UniProt Gene Name: Tbp
• UniProt Synonym Gene Names: Tfiid
• UniProt Entry Name: TBP_MOUSE

Uniprot Description

TBP: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1. Interacts with CITED2. Interacts with ATF7IP. Interacts with HIV-1 Tat. Widely expressed, with levels highest in the testis and ovary. Belongs to the TBP family.

Protein type: Transcription initiation complex; DNA-binding; Nuclear receptor co-regulator

Cellular Component: transcription factor TFIID complex; male pronucleus; pronucleus; cytoplasm; female pronucleus; RNA polymerase I transcription factor complex; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription from RNA polymerase III promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transcription initiation