TBL1XR1 sirna

TBL1XR1 siRNA (Mouse)

Cat. Num. AAA8235001

• Gene Names: Tbl1xr1; C21; DC42; Ira1; TBLR1; AW539987; 8030499H02Rik; A630076E03Rik; C230089I12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBL1XR1; TBL1XR1 siRNA (Mouse); IRA1; TBLR1; F-box-like/WD repeat-containing protein TBL1XR1; Nuclear receptor corepressor/HDAC3 complex subunit TBLR1; TBL1-related protein 1; Transducin beta-like 1X-related protein 1; TBL1XR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TBL1XR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 514

• Applicable Applications for TBL1XR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TBL1XR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBL1XR1 sirna

siRNA to inhibit TBL1XR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543001
• NCBI GeneID: 81004
• NCBI Accession #: NP_109657.2
• NCBI GenBank Nucleotide #: NM_030732.3
• UniProt Accession #: Q8BHJ5; Q3UWL6; Q8CBG4; Q8VEG3; Q9EQD4
• Molecular Weight: 55,661 Da
• NCBI Official Full Name: F-box-like/WD repeat-containing protein TBL1XR1
• NCBI Official Synonym Full Names: transducin (beta)-like 1X-linked receptor 1
• NCBI Official Symbol: Tbl1xr1
• NCBI Official Synonym Symbols: C21; DC42; Ira1; TBLR1; AW539987; 8030499H02Rik; A630076E03Rik; C230089I12Rik
• NCBI Protein Information: F-box-like/WD repeat-containing protein TBL1XR1
• UniProt Protein Name: F-box-like/WD repeat-containing protein TBL1XR1
• Protein Family: F-box-like/WD repeat-containing protein
• UniProt Gene Name: Tbl1xr1
• UniProt Synonym Gene Names: Ira1; Tblr1
• UniProt Entry Name: TBL1R_MOUSE

Uniprot Description

TBL1XR1: F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Probable component of some E3 ubiquitin ligase complex. Interacts with histones H2B and H4. Ubiquitous. Belongs to the WD repeat EBI family.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: histone deacetylase complex; nucleus

Molecular Function: protein binding; DNA binding; transcription corepressor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; response to dietary excess; transcription, DNA-dependent; regulation of cAMP metabolic process; multicellular organism growth; histone deacetylation; chromatin modification; regulation of transcription, DNA-dependent; regulation of gene expression; white fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; lipid catabolic process