TAF3 sirna
TAF3 siRNA (Mouse)
Gene Names
Taf3; 140kDa; TAF140; AW539625; TAFII140; TAFII-140; mTAFII140; 4933439M23Rik
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
TAF3; TAF3 siRNA (Mouse); Transcription initiation factor TFIID subunit 3; 140 kDa TATA box-binding protein-associated factor; TBP-associated factor 3; Transcription initiation factor TFIID 140 kDa subunit; TAF(II)140; TAF140; TAFII-140; TAFII140; TAF3 sirna
Host
Synthetic
Reactivity
Mouse
Specificity
TAF3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
932
Applicable Applications for TAF3 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TAF3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for TAF3 sirna
siRNA to inhibit TAF3 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,794 Da
NCBI Official Full Name
transcription initiation factor TFIID subunit 3
NCBI Official Synonym Full Names
TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor
NCBI Official Symbol
Taf3
NCBI Official Synonym Symbols
140kDa; TAF140; AW539625; TAFII140; TAFII-140; mTAFII140; 4933439M23Rik
NCBI Protein Information
transcription initiation factor TFIID subunit 3
UniProt Protein Name
Transcription initiation factor TFIID subunit 3
Protein Family
UniProt Gene Name
Taf3
UniProt Synonym Gene Names
TAF(II)140; TAF140; TAFII-140; TAFII140
UniProt Entry Name
TAF3_MOUSE
Uniprot Description
TAF140: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. Belongs to the TAF3 family.
Protein type: Transcription initiation complex
Cellular Component: transcription factor TFIID complex; nucleus
Molecular Function: protein binding; zinc ion binding; p53 binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription factor activity; maintenance of protein localization in nucleus; negative regulation of transcription from RNA polymerase II promoter
