STK39 sirna

STK39 siRNA (Mouse)

Cat. Num. AAA8226265

• Gene Names: Stk39; DCHT; Rnl5; SPAK; RF005; AW227544; AW556857
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: STK39; STK39 siRNA (Mouse); SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; Serine/threonine-protein kinase 39; STK39 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: STK39 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 556

• Applicable Applications for STK39 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse STK39 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for STK39 sirna

siRNA to inhibit STK39 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8394347
• NCBI GeneID: 53416
• NCBI Accession #: NP_058562.1
• NCBI GenBank Nucleotide #: NM_016866.2
• UniProt Accession #: Q9Z1W9; Q80W13
• Molecular Weight: 60,320 Da
• NCBI Official Full Name: STE20/SPS1-related proline-alanine-rich protein kinase
• NCBI Official Synonym Full Names: serine/threonine kinase 39
• NCBI Official Symbol: Stk39
• NCBI Official Synonym Symbols: DCHT; Rnl5; SPAK; RF005; AW227544; AW556857
• NCBI Protein Information: STE20/SPS1-related proline-alanine-rich protein kinase
• UniProt Protein Name: STE20/SPS1-related proline-alanine-rich protein kinase
• Protein Family: STE20/SPS1-related proline-alanine-rich protein kinase
• UniProt Gene Name: Stk39
• UniProt Synonym Gene Names: Spak; Ste-20-related kinase
• UniProt Entry Name: STK39_MOUSE

Uniprot Description

STLK3: May act as a mediator of stress-activated signals. The phosphorylated form forms a complex with WNK2. Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Protein kinase, STE; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; FRAY subfamily

Cellular Component: cytoskeleton; membrane; basolateral plasma membrane; apical plasma membrane; cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-serine phosphorylation; negative regulation of protein amino acid phosphorylation; regulation of blood pressure; regulation of inflammatory response; positive regulation of potassium ion transport; peptidyl-threonine phosphorylation; phosphorylation; protein amino acid phosphorylation