ST3GAL3 sirna

ST3GAL3 siRNA (Mouse)

Cat. Num. AAA8203407

• Gene Names: St3gal3; ST3N; Siat3; Siat6; ST3GalIII
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ST3GAL3; ST3GAL3 siRNA (Mouse); SIAT3; SIAT6; CMP-N-acetylneuraminate-beta-1.4-galactoside alpha-2.3-sialyltransferase; Beta-galactoside alpha-2.3-sialyltransferase 3; Alpha 2.3-ST 3; Gal beta-1.3(4) GlcNAc alpha-2.3 sialyltransferase; N-acetyllactosaminide alpha-2.3-sialyltransferase; ST3Gal III; ST3GalIII; ST3N; Sialyltransferase 6; ST3GAL3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ST3GAL3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 374

• Applicable Applications for ST3GAL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ST3GAL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ST3GAL3 sirna

siRNA to inhibit ST3GAL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239937471
• NCBI GeneID: 20441
• NCBI Accession #: NP_001155246.1
• NCBI GenBank Nucleotide #: NM_001161774.2
• UniProt Accession #: P97325; Q922X5
• Molecular Weight: 42,132 Da
• NCBI Official Full Name: CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase isoform a
• NCBI Official Synonym Full Names: ST3 beta-galactoside alpha-2,3-sialyltransferase 3
• NCBI Official Symbol: St3gal3
• NCBI Official Synonym Symbols: ST3N; Siat3; Siat6; ST3GalIII
• NCBI Protein Information: CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
• UniProt Protein Name: CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
• Protein Family: CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
• UniProt Gene Name: St3gal3
• UniProt Synonym Gene Names: Siat3; Siat6; Alpha 2,3-ST 3; ST3GalIII
• UniProt Entry Name: SIAT6_MOUSE

Uniprot Description

ST3GAL3: Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta- 1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha- 2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3- GalNAc. Defects in ST3GAL3 are the cause of mental retardation autosomal recessive type 12 (MRT12). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the glycosyltransferase 29 family. 26 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.99.6; Glycan Metabolism - keratan sulfate biosynthesis

Cellular Component: Golgi apparatus; membrane; integral to membrane; integral to Golgi membrane

Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; sialyltransferase activity; N-acetyllactosaminide alpha-2,3-sialyltransferase activity; beta-galactoside alpha-2,3-sialyltransferase activity

Biological Process: protein amino acid glycosylation