SOCS1 sirna

SOCS1 siRNA (Mouse)

Cat. Num. AAA8221326

• Gene Names: Socs1; JAB; Cish1; Cish7; SSI-1; SOCS-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SOCS1; SOCS1 siRNA (Mouse); CISH1; SSI1; Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; SOCS1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SOCS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 212

• Applicable Applications for SOCS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SOCS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SOCS1 sirna

siRNA to inhibit SOCS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 409971432
• NCBI GeneID: 12703
• NCBI Accession #: NP_001258532.1
• NCBI GenBank Nucleotide #: NM_001271603.1
• UniProt Accession #: O35716; O35960; Q3U3L0; A2RT46
• Molecular Weight: 23,715 Da
• NCBI Official Full Name: suppressor of cytokine signaling 1
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 1
• NCBI Official Symbol: Socs1
• NCBI Official Synonym Symbols: JAB; Cish1; Cish7; SSI-1; SOCS-1
• NCBI Protein Information: suppressor of cytokine signaling 1
• UniProt Protein Name: Suppressor of cytokine signaling 1
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: Socs1
• UniProt Synonym Gene Names: Cish1; Ssi1; SOCS-1; JAB; SSI-1
• UniProt Entry Name: SOCS1_MOUSE

Uniprot Description

SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Protein type: Inhibitor

Cellular Component: cytoplasm; intracellular; cytoplasmic vesicle; nucleus

Molecular Function: insulin-like growth factor receptor binding; protein binding; protein kinase inhibitor activity; kinase inhibitor activity; protein kinase binding

Biological Process: fat cell differentiation; regulation of protein amino acid phosphorylation; regulation of cytokine secretion; cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; JAK-STAT cascade; regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of signal transduction; regulation of JAK-STAT cascade; regulation of growth; negative regulation of catalytic activity; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of protein kinase activity; negative regulation of JAK-STAT cascade