SMPD2 sirna

SMPD2 siRNA (Mouse)

Cat. Num. AAA8211848

• Gene Names: Smpd2; nSMase; nSMase1; AW108287
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMPD2; SMPD2 siRNA (Mouse); Sphingomyelin phosphodiesterase 2; Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase; SMPD2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SMPD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 419

• Applicable Applications for SMPD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SMPD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMPD2 sirna

siRNA to inhibit SMPD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678031
• NCBI GeneID: 20598
• NCBI Accession #: NP_033239.1
• NCBI GenBank Nucleotide #: NM_009213.2
• UniProt Accession #: O70572
• Molecular Weight: 47,467 Da
• NCBI Official Full Name: sphingomyelin phosphodiesterase 2
• NCBI Official Synonym Full Names: sphingomyelin phosphodiesterase 2, neutral
• NCBI Official Symbol: Smpd2
• NCBI Official Synonym Symbols: nSMase; nSMase1; AW108287
• NCBI Protein Information: sphingomyelin phosphodiesterase 2
• UniProt Protein Name: Sphingomyelin phosphodiesterase 2
• UniProt Gene Name: Smpd2
• UniProt Synonym Gene Names: Lyso-PAF-PLC; N-SMase; nSMase
• UniProt Entry Name: NSMA_MOUSE

NCBI Description

This gene encodes a protein with similarity to the human nSMase1 protein. In humans, the nSMase1 protein was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]

Uniprot Description

SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family.

Protein type: Membrane protein, multi-pass; Lipid Metabolism - sphingolipid; Membrane protein, integral; Phosphodiesterase; EC 3.1.4.12

Cellular Component: membrane; integral to membrane; intracellular; caveola

Molecular Function: sphingomyelin phosphodiesterase activity; hydrolase activity; metal ion binding

Biological Process: sphingolipid metabolic process; response to mechanical stimulus; positive regulation of apoptosis; ceramide biosynthetic process; lipid metabolic process