SMC3 sirna

SMC3 siRNA (Mouse)

Cat. Num. AAA8220454

• Gene Names: Smc3; HCAP; SmcD; Cspg6; Mmip1; SMC-3; Bamacan
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMC3; SMC3 siRNA (Mouse); BAM; BMH; CSPG6; MMIP1; SMC3L1; SMCD; Structural maintenance of chromosomes protein 3; SMC protein 3; SMC-3; Basement membrane-associated chondroitin proteoglycan; Bamacan; Chondroitin sulfate proteoglycan 6; Chromosome segregation protein SmcD; Mad member-interacting protein 1; SMC3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SMC3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1217

• Applicable Applications for SMC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SMC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMC3 sirna

siRNA to inhibit SMC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 36031035
• NCBI GeneID: 13006
• NCBI Accession #: NP_031816.2
• NCBI GenBank Nucleotide #: NM_007790.3
• UniProt Accession #: Q9CW03; O35667; Q9QUS3
• Molecular Weight: 141,556 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: Smc3
• NCBI Official Synonym Symbols: HCAP; SmcD; Cspg6; Mmip1; SMC-3; Bamacan
• NCBI Protein Information: structural maintenance of chromosomes protein 3
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: Smc3
• UniProt Synonym Gene Names: Bam; Bmh; Cspg6; Mmip1; Smc3l1; Smcd; SMC protein 3; SMC-3; Bamacan
• UniProt Entry Name: SMC3_MOUSE

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: DNA repair, damage; Cell cycle regulation

Cellular Component: nucleoplasm; lateral element; spindle pole; cohesin core heterodimer; nuclear matrix; cytoplasm; basement membrane; chromosome; chromatin; nucleus; chromosome, pericentric region

Molecular Function: dynein binding; protein binding; protein heterodimerization activity; nucleotide binding; chromatin binding; ATP binding

Biological Process: meiosis; mitosis; stem cell maintenance; mitotic sister chromatid cohesion; sister chromatid cohesion; negative regulation of DNA endoreduplication; signal transduction; DNA repair; cell cycle; chromosome organization and biogenesis; meiotic cell cycle; cell division; response to DNA damage stimulus; regulation of DNA replication