SMARCA2 sirna

SMARCA2 siRNA (Mouse)

Cat. Num. AAA8211895

• Gene Names: Smarca2; brm; Snf2l2; brahma; SNF2alpha; 2610209L14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMARCA2; SMARCA2 siRNA (Mouse); BAF190B; BRM; SNF2A; SNF2L2; Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; Protein brahma homolog; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SMARCA2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SMARCA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1583

• Applicable Applications for SMARCA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SMARCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMARCA2 sirna

siRNA to inhibit SMARCA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51593084
• NCBI GeneID: 67155
• NCBI Accession #: NP_035546.2
• NCBI GenBank Nucleotide #: NM_011416.2
• UniProt Accession #: Q6DIC0
• Molecular Weight: 180,253 Da
• NCBI Official Full Name: probable global transcription activator SNF2L2 isoform 1
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• NCBI Official Symbol: Smarca2
• NCBI Official Synonym Symbols: brm; Snf2l2; brahma; SNF2alpha; 2610209L14Rik
• NCBI Protein Information: probable global transcription activator SNF2L2
• UniProt Protein Name: Probable global transcription activator SNF2L2
• Protein Family: Probable global transcription activator
• UniProt Gene Name: Smarca2
• UniProt Synonym Gene Names: BAF190B
• UniProt Entry Name: SMCA2_MOUSE

Uniprot Description

SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: Helicase; EC 3.6.1.-; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 3.6.4.-

Cellular Component: nucleoplasm; SWI/SNF complex; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus

Molecular Function: protein binding; hydrolase activity, acting on acid anhydrides; DNA binding; histone binding; hydrolase activity; ATPase activity; nucleotide binding; chromatin binding; helicase activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: chromatin remodeling; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; nervous system development; nucleosome assembly; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent