SLC52A3 sirna

SLC52A3 siRNA (Mouse)

Cat. Num. AAA8211930

• Gene Names: Slc52a3; RFT2; 2310046K01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC52A3; SLC52A3 siRNA (Mouse); RFT2; Solute carrier family 52. riboflavin transporter. member 3; Riboflavin transporter 2; SLC52A3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC52A3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 460

• Applicable Applications for SLC52A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC52A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC52A3 sirna

siRNA to inhibit SLC52A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 258679447
• NCBI GeneID: 69698
• NCBI Accession #: NP_001158291.1
• NCBI GenBank Nucleotide #: NM_001164819.1
• UniProt Accession #: Q9D6X5; Q3TDJ6; Q3U328; Q8CE36; Q91WB9; A2AQU3
• Molecular Weight: 26,770 Da
• NCBI Official Full Name: solute carrier family 52, riboflavin transporter, member 3 isoform 1
• NCBI Official Synonym Full Names: solute carrier protein family 52, member 3
• NCBI Official Symbol: Slc52a3
• NCBI Official Synonym Symbols: RFT2; 2310046K01Rik
• NCBI Protein Information: solute carrier family 52, riboflavin transporter, member 3
• UniProt Protein Name: Solute carrier family 52, riboflavin transporter, member 3
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc52a3
• UniProt Synonym Gene Names: Rft2; RFVT3; RFT2
• UniProt Entry Name: S52A3_MOUSE

Uniprot Description

SLC52A3: Riboflavin transporter. Riboflavin transport is Na(+)- independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride. Defects in SLC52A3 are the cause of Brown-Vialetto-Van Laere syndrome type 1 (BVVLS1). A rare autosomal recessive neurologic disorder characterized by sensorineural hearing loss and a variety of cranial nerve palsies, which develop over a relatively short period of time in a previously healthy individual. Sensorineural hearing loss may precede the neurological signs. The course is invariably progressive, but the rate of decline is variable within and between families. With disease evolution, long tract signs, lower motor neuron signs, cerebellar ataxia, and lower cranial nerve (III-VI) palsies develop, giving rise to a complex picture resembling amyotrophic lateral sclerosis. Diaphragmatic weakness and respiratory compromise are some of the most distressing features, leading to recurrent chest infections and respiratory failure, which are often the cause of patients' demise. Defects in SLC52A3 are the cause of Fazio-Londe disease (FALOND). A rare neurological disease characterized by progressive weakness of the muscles innervated by cranial nerves of the lower brain stem. It may present in childhood with severe neurological deterioration with hypotonia, respiratory insufficiency leading to premature death, or later in life with bulbar weakness which progresses to involve motor neurons throughout the neuroaxis. Clinical manifestations include dysarthria, dysphagia, facial weakness, tongue weakness, and fasciculations of the tongue and facial muscles. Belongs to the riboflavin transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; riboflavin transporter activity

Biological Process: sensory perception of sound; transport; riboflavin transport