SLC25A13 sirna

SLC25A13 siRNA (Mouse)

Cat. Num. AAA8228140

• Gene Names: Slc25a13; Ctrn; AI785475
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC25A13; SLC25A13 siRNA (Mouse); ARALAR2; Calcium-binding mitochondrial carrier protein Aralar2; Citrin; Mitochondrial aspartate glutamate carrier 2; Solute carrier family 25 member 13; SLC25A13 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC25A13 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 675

• Applicable Applications for SLC25A13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC25A13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC25A13 sirna

siRNA to inhibit SLC25A13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 294832028
• NCBI GeneID: 50799
• NCBI Accession #: NP_001171043.1
• NCBI GenBank Nucleotide #: NM_001177572.1
• UniProt Accession #: Q9QXX4; Q9CZF6; Q9DCF5
• Molecular Weight: 74,467 Da
• NCBI Official Full Name: calcium-binding mitochondrial carrier protein Aralar2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
• NCBI Official Symbol: Slc25a13
• NCBI Official Synonym Symbols: Ctrn; AI785475
• NCBI Protein Information: calcium-binding mitochondrial carrier protein Aralar2
• UniProt Protein Name: Calcium-binding mitochondrial carrier protein Aralar2
• Protein Family: Calcium-binding mitochondrial carrier protein
• UniProt Gene Name: Slc25a13
• UniProt Synonym Gene Names: Aralar2
• UniProt Entry Name: CMC2_MOUSE

Uniprot Description

SLC25A13: Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2). Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years. Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD). NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: L-aspartate transmembrane transporter activity; metal ion binding; calcium ion binding; L-glutamate transmembrane transporter activity

Biological Process: L-glutamate transport; transport; ATP biosynthetic process; cellular respiration; aspartate transport; response to calcium ion; malate-aspartate shuttle; transmembrane transport