SERPINI1 sirna

SERPINI1 siRNA (Mouse)

Cat. Num. AAA8235432

• Gene Names: Serpini1; Ns; PI12; PI-12; Spi17; AI837402
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SERPINI1; SERPINI1 siRNA (Mouse); PI12; SPI17; Neuroserpin; Peptidase inhibitor 12; PI-12; Serine protease inhibitor 17; Serpin I1; SERPINI1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SERPINI1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for SERPINI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SERPINI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SERPINI1 sirna

siRNA to inhibit SERPINI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678091
• NCBI GeneID: 20713
• NCBI Accession #: NP_033276.1
• NCBI GenBank Nucleotide #: NM_009250.2
• UniProt Accession #: O35684; Q543F7; Q922U6
• Molecular Weight: 46,348 Da
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade I, member 1
• NCBI Official Symbol: Serpini1
• NCBI Official Synonym Symbols: Ns; PI12; PI-12; Spi17; AI837402
• NCBI Protein Information: neuroserpin
• UniProt Protein Name: Neuroserpin
• Protein Family: Neuroserpin
• UniProt Gene Name: Serpini1
• UniProt Synonym Gene Names: Pi12; Spi17; PI-12
• UniProt Entry Name: NEUS_MOUSE

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: regulation of cell adhesion; negative regulation of peptidase activity