SCYL2 sirna

SCYL2 siRNA (Mouse)

Cat. Num. AAA8231947

• Gene Names: Scyl2; CVAK104; AU015688; AW536445; BC030932; D10Ertd802e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCYL2; SCYL2 siRNA (Mouse); CVAK104; D10ERTD802E; SCY1-like protein 2; Coated vesicle-associated kinase of 104 kDa; SCYL2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SCYL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 930

• Applicable Applications for SCYL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCYL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCYL2 sirna

siRNA to inhibit SCYL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 37574121
• NCBI GeneID: 213326
• NCBI Accession #: NP_932138.1
• NCBI GenBank Nucleotide #: NM_198021.2
• UniProt Accession #: Q8CFE4; Q3UT57; Q3UWU9; Q8K0M4
• Molecular Weight: 55,421 Da
• NCBI Official Full Name: SCY1-like protein 2 isoform 1
• NCBI Official Synonym Full Names: SCY1-like 2 (S. cerevisiae)
• NCBI Official Symbol: Scyl2
• NCBI Official Synonym Symbols: CVAK104; AU015688; AW536445; BC030932; D10Ertd802e
• NCBI Protein Information: SCY1-like protein 2
• UniProt Protein Name: SCY1-like protein 2
• Protein Family: SCY1-like protein
• UniProt Gene Name: Scyl2
• UniProt Synonym Gene Names: Cvak104; D10Ertd802e
• UniProt Entry Name: SCYL2_MOUSE

Uniprot Description

SCYL2: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. Belongs to the protein kinase superfamily.

Protein type: Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; SCY1 family

Cellular Component: Golgi apparatus; membrane; cell; cytoplasm; cytoplasmic vesicle; intracellular; endosome

Molecular Function: protein binding; transferase activity, transferring phosphorus-containing groups; ATP binding; receptor binding; protein kinase activity

Biological Process: positive regulation of receptor internalization; endosome to lysosome transport; protein amino acid phosphorylation