SCN4A sirna

SCN4A siRNA (Mouse)

Cat. Num. AAA8215981

• Gene Names: Scn4a; mH2; SkM1; Nav1.4
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCN4A; SCN4A siRNA (Mouse); Sodium channel protein type 4 subunit alpha; Sodium channel protein skeletal muscle subunit alpha; Sodium channel protein type IV subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.4; SCN4A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SCN4A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,841

• Applicable Applications for SCN4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCN4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCN4A sirna

siRNA to inhibit SCN4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 134948032
• NCBI GeneID: 110880
• NCBI Accession #: NP_573462.2
• NCBI GenBank Nucleotide #: NM_133199.2
• UniProt Accession #: Q9ER60; A2VDE9; B1ARK0
• Molecular Weight: 208,798 Da
• NCBI Official Full Name: sodium channel protein type 4 subunit alpha
• NCBI Official Synonym Full Names: sodium channel, voltage-gated, type IV, alpha
• NCBI Official Symbol: Scn4a
• NCBI Official Synonym Symbols: mH2; SkM1; Nav1.4
• NCBI Protein Information: sodium channel protein type 4 subunit alpha
• UniProt Protein Name: Sodium channel protein type 4 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: Scn4a
• UniProt Entry Name: SCN4A_MOUSE

Uniprot Description

SCN4A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle. Defects in SCN4A are the cause of paramyotonia congenita of von Eulenburg (PMC). PMC is an autosomal dominant channelopathy characterized by myotonia, increased by exposure to cold, intermittent flaccid paresis, not necessarily dependent on cold or myotonia, lability of serum potassium, nonprogressive nature and lack of atrophy or hypertrophy of muscles. In some patients, myotonia is not increased by cold exposure (paramyotonia without cold paralysis). Patients may have a combination phenotype of PMC and HYPP. Defects in SCN4A are a cause of periodic paralysis hypokalemic type 2 (HOKPP2). It is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels. Defects in SCN4A are the cause of periodic paralysis hyperkalemic (HYPP). HYPP is an autosomal dominant channelopathy characterized by episodic flaccid generalized muscle weakness associated with high levels of serum potassium. Concurrence of myotonia is found in HYPP patients. Defects in SCN4A are the cause of periodic paralysis normokalemic (NKPP). NKPP is a disorder closely related to hyperkalemic periodic paralysis, but marked by a lack of alterations in potassium levels during attacks of muscle weakness. Defects in SCN4A are the cause of myotonia SCN4A-related (MYOSCN4A). Myotonia is characterized by sustained muscle tensing that prevents muscles from relaxing normally. Myotonia causes muscle stiffness that can interfere with movement. In some people the stiffness is very mild, while in other cases it may be severe enough to interfere with walking, running, and other activities of daily life. MYOSCN4A is a phenotypically highly variable myotonia aggravated by potassium loading, and often by cold. MYOSCN4A includes myotonia permanens and myotonia fluctuans. In myotonia permanens, the myotonia is generalized and there is a hypertrophy of the muscle, particularly in the neck and the shoulder. Attacks of severe muscle stiffness of the thoracic muscles may be life threatening due to impaired ventilation. In myotonia fluctuans, the muscle stiffness may fluctuate from day to day, provoked by exercise. Defects in SCN4A are the cause of a congenital myasthenic syndrome acetazolamide-responsive (CMSAR). A congenital myasthenic syndrome associated with fatigable generalized weakness and recurrent attacks of respiratory and bulbar paralysis since birth. The fatigable weakness involves lid- elevator, external ocular, facial, limb and truncal muscles and an decremental response of the compound muscle action potential on repetitive stimulation. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.

Protein type: Membrane protein, multi-pass; Channel, sodium; Membrane protein, integral

Cellular Component: voltage-gated sodium channel complex; membrane; integral to membrane; plasma membrane

Molecular Function: voltage-gated sodium channel activity; ion channel activity; voltage-gated ion channel activity; sodium channel activity

Biological Process: transport; sodium ion transport; generation of action potential; ion transport; choline transport; transmembrane transport; cation transport; potassium ion transport