RUNX2 sirna

RUNX2 siRNA (Mouse)

Cat. Num. AAA8204852

• Gene Names: Runx2; Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RUNX2; RUNX2 siRNA (Mouse); AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; RUNX2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RUNX2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 528

• Applicable Applications for RUNX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RUNX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RUNX2 sirna

siRNA to inhibit RUNX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 225690526
• NCBI GeneID: 12393
• NCBI Accession #: NP_001139510.1
• NCBI GenBank Nucleotide #: NM_001146038.2
• UniProt Accession #: Q08775; O35183; Q08776; Q9JLN0; Q9QUQ6; Q9QY29; Q9R0U4; Q9Z2J7
• Molecular Weight: 52,285 Da
• NCBI Official Full Name: runt-related transcription factor 2 isoform 1
• NCBI Official Synonym Full Names: runt related transcription factor 2
• NCBI Official Symbol: Runx2
• NCBI Official Synonym Symbols: Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a
• NCBI Protein Information: runt-related transcription factor 2
• UniProt Protein Name: Runt-related transcription factor 2
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: Runx2
• UniProt Synonym Gene Names: Aml3; Cbfa1; Osf2; Pebp2a; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A
• UniProt Entry Name: RUNX2_MOUSE

Uniprot Description

AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; transcription factor binding; ATP binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell maturation; neuron differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification; regulation of osteoblast differentiation; transcription, DNA-dependent; regulation of fibroblast growth factor receptor signaling pathway; stem cell differentiation; embryonic cranial skeleton morphogenesis; chondrocyte development; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of ossification; osteoblast fate commitment