RPS6KA1 sirna

RPS6KA1 siRNA (Mouse)

Cat. Num. AAA8216709

• Gene Names: Rps6ka1; Rsk; Rsk1; Rsk-1; p90S6K; p90rsk; p90Rsk1; p90-Rsk1; Mapkapk-1a; S6K-alpha-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPS6KA1; RPS6KA1 siRNA (Mouse); MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RPS6KA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 724

• Applicable Applications for RPS6KA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RPS6KA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPS6KA1 sirna

siRNA to inhibit RPS6KA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 550822351
• NCBI GeneID: 20111
• NCBI Accession #: NP_001272434.1
• NCBI GenBank Nucleotide #: NM_001285505.1
• UniProt Accession #: P18653
• Molecular Weight: 81,595 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase polypeptide 1
• NCBI Official Symbol: Rps6ka1
• NCBI Official Synonym Symbols: Rsk; Rsk1; Rsk-1; p90S6K; p90rsk; p90Rsk1; p90-Rsk1; Mapkapk-1a; S6K-alpha-1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6ka1
• UniProt Synonym Gene Names: Mapkapk1a; Rsk1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_MOUSE

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Translation; Protein kinase, AGC; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; spindle; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; nucleotide binding; magnesium ion binding; kinase activity; ribosomal protein S6 kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; cellular response to stress; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; protein amino acid phosphorylation; phosphorylation; negative regulation of apoptosis