RPE65 sirna

RPE65 siRNA (Mouse)

Cat. Num. AAA8206173

• Gene Names: Rpe65; LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPE65; RPE65 siRNA (Mouse); Retinoid isomerohydrolase; All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase; RPE65 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RPE65 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 533

• Applicable Applications for RPE65 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RPE65 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPE65 sirna

siRNA to inhibit RPE65 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 147902089
• NCBI GeneID: 19892
• NCBI Accession #: NP_084263.2
• NCBI GenBank Nucleotide #: NM_029987.2
• UniProt Accession #: Q91ZQ5; Q8VHP2; A1L3D1; E9QNS6; H9KUX9
• Molecular Weight: 61,085 Da
• NCBI Official Full Name: retinoid isomerohydrolase
• NCBI Official Synonym Full Names: retinal pigment epithelium 65
• NCBI Official Symbol: Rpe65
• NCBI Official Synonym Symbols: LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
• NCBI Protein Information: retinoid isomerohydrolase
• UniProt Protein Name: Retinoid isomerohydrolase
• UniProt Gene Name: Rpe65
• UniProt Entry Name: RPE65_MOUSE

Uniprot Description

RPE65: Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in RPE65 are a cause of autosomal dominant retinitis pigmentosa with choroidal involvement (PubMed:21654732). Affected individuals show reduction of central vision, constriction of visual fields, night blindness and chorioretinal atrophy. Belongs to the carotenoid oxygenase family.

Protein type: EC 3.1.1.64; Cofactor and Vitamin Metabolism - retinol

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane

Molecular Function: hydrolase activity; retinal isomerase activity; metal ion binding; isomerase activity

Biological Process: retinal homeostasis; retinal metabolic process; visual perception; retinol metabolic process; retina development in camera-type eye; response to stimulus; insulin receptor signaling pathway; detection of light stimulus involved in visual perception; regulation of rhodopsin gene expression; retina morphogenesis in camera-type eye