RHOQ sirna

RHOQ siRNA (Mouse)

Cat. Num. AAA8208542

• Gene Names: Rhoq; Arhq; Tc10; TC10A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RHOQ; RHOQ siRNA (Mouse); ARHQ; TC10; Rho-related GTP-binding protein RhoQ; Ras-like protein TC10; RHOQ sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RHOQ siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 205

• Applicable Applications for RHOQ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RHOQ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RHOQ sirna

siRNA to inhibit RHOQ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34328361
• NCBI GeneID: 104215
• NCBI Accession #: NP_663466.2
• NCBI GenBank Nucleotide #: NM_145491.2
• UniProt Accession #: Q8R527; Q7TNC0; Q80VH4
• Molecular Weight: 22,645 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoQ
• NCBI Official Synonym Full Names: ras homolog gene family, member Q
• NCBI Official Symbol: Rhoq
• NCBI Official Synonym Symbols: Arhq; Tc10; TC10A
• NCBI Protein Information: rho-related GTP-binding protein RhoQ
• UniProt Protein Name: Rho-related GTP-binding protein RhoQ
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: Rhoq
• UniProt Synonym Gene Names: Arhq; Tc10
• UniProt Entry Name: RHOQ_MOUSE

Uniprot Description

RHOQ: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. Belongs to the small GTPase superfamily. Rho family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, Rho

Cellular Component: membrane; cytoplasm; plasma membrane; actin filament; intracellular; lipid raft

Molecular Function: GTPase activity; protein binding; GTP binding; nucleotide binding; GBD domain binding; profilin binding

Biological Process: positive regulation of filopodium formation; positive regulation of glucose import; regulation of cell shape; negative regulation of glucose import; cellular response to insulin stimulus; metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cortical actin cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis