RECQL4 sirna
RECQL4 siRNA (Mouse)
Gene Names
Recql4; RecQ4
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
RECQL4; RECQL4 siRNA (Mouse); RECQ4; ATP-dependent DNA helicase Q4; DNA helicase. RecQ-like type 4; RecQ4; RecQ protein-like 4; RECQL4 sirna
Host
Synthetic
Reactivity
Mouse
Specificity
RECQL4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
1,266
Applicable Applications for RECQL4 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RECQL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for RECQL4 sirna
siRNA to inhibit RECQL4 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,415 Da
NCBI Official Full Name
ATP-dependent DNA helicase Q4
NCBI Official Synonym Full Names
RecQ protein-like 4
NCBI Official Symbol
Recql4
NCBI Official Synonym Symbols
RecQ4
NCBI Protein Information
ATP-dependent DNA helicase Q4
UniProt Protein Name
ATP-dependent DNA helicase Q4
Protein Family
UniProt Gene Name
Recql4
UniProt Synonym Gene Names
Recq4; RecQ4
UniProt Entry Name
RECQ4_MOUSE
Uniprot Description
RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily.
Protein type: EC 3.6.4.12; Helicase
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: nucleic acid binding; ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; hydrolase activity; metal ion binding; nucleotide binding; ATP-dependent helicase activity; bubble DNA binding; helicase activity; ATP binding
Biological Process: pigmentation; skeletal morphogenesis; positive regulation of cell proliferation; DNA strand renaturation; negative regulation of sister chromatid cohesion; DNA replication; skeletal development; DNA duplex unwinding; DNA recombination
Research Articles on RECQL4
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Product Notes
The RECQL4 recql4 (Catalog #AAA8237521) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The RECQL4 siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RECQL4 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the RECQL4 recql4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RECQL4, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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