RBBP7 sirna

RBBP7 siRNA (Mouse)

Cat. Num. AAA8220591

• Gene Names: Rbbp7; mRbAp46; AA409861; AI173248; AU019541; BB114024
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RBBP7; RBBP7 siRNA (Mouse); RBAP46; Histone-binding protein RBBP7; Histone acetyltransferase type B subunit 2; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7; Retinoblastoma-binding protein p46; RBBP7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RBBP7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 425

• Applicable Applications for RBBP7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RBBP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RBBP7 sirna

siRNA to inhibit RBBP7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157909799
• NCBI GeneID: 245688
• NCBI Accession #: NP_033057.3
• NCBI GenBank Nucleotide #: NM_009031.3
• UniProt Accession #: Q60973; Q3UX20; A2AFJ0
• Molecular Weight: 47,790 Da
• NCBI Official Full Name: histone-binding protein RBBP7
• NCBI Official Synonym Full Names: retinoblastoma binding protein 7
• NCBI Official Symbol: Rbbp7
• NCBI Official Synonym Symbols: mRbAp46; AA409861; AI173248; AU019541; BB114024
• NCBI Protein Information: histone-binding protein RBBP7
• UniProt Protein Name: Histone-binding protein RBBP7
• Protein Family: Histone-binding protein
• UniProt Gene Name: Rbbp7
• UniProt Synonym Gene Names: Rbap46; RBBP-7
• UniProt Entry Name: RBBP7_MOUSE

Uniprot Description

RBBP7: Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the type B histone acetyltransferase (HAT) complex, composed of RBBP7 and HAT1. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein- binding domain of the retinoblastoma protein (RB1). Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Interacts with BRCA1, HDAC7 and SUV39H1. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Cellular Component: ESC/E(Z) complex; NuRD complex; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; chromatin modification; negative regulation of transcription, DNA-dependent; DNA replication