RALA sirna

RALA siRNA (Mouse)

Cat. Num. AAA8212542

• Gene Names: Rala; Ral; Rasl1; AW322615; 3010001O15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RALA; RALA siRNA (Mouse); RAL; RAL-A; Ras-related protein Ral-A; RALA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RALA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 206

• Applicable Applications for RALA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RALA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RALA sirna

siRNA to inhibit RALA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34328471
• NCBI GeneID: 56044
• NCBI Accession #: NP_062364.3
• NCBI GenBank Nucleotide #: NM_019491.5
• UniProt Accession #: P63321; P05810
• Molecular Weight: 23,553 Da
• NCBI Official Full Name: ras-related protein Ral-A
• NCBI Official Synonym Full Names: v-ral simian leukemia viral oncogene homolog A (ras related)
• NCBI Official Symbol: Rala
• NCBI Official Synonym Symbols: Ral; Rasl1; AW322615; 3010001O15Rik
• NCBI Protein Information: ras-related protein Ral-A
• UniProt Protein Name: Ras-related protein Ral-A
• Protein Family: Ras-related protein
• UniProt Gene Name: Rala
• UniProt Synonym Gene Names: Ral; Ral-a
• UniProt Entry Name: RALA_MOUSE

Uniprot Description

RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Ras

Cellular Component: focal adhesion; cell surface; membrane; endocytic vesicle; plasma membrane; myelin sheath; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; myosin binding; GTP binding; Edg-2 lysophosphatidic acid receptor binding; ubiquitin protein ligase binding; nucleotide binding; ATPase binding

Biological Process: positive regulation of filopodium formation; regulation of exocytosis; exocytosis; lipid raft localization; cell division; small GTPase mediated signal transduction; actin cytoskeleton reorganization; Ras protein signal transduction; cytokinesis; cell cycle; signal transduction