RAD1 sirna

RAD1 siRNA (Mouse)

Cat. Num. AAA8215322

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAD1; RAD1 siRNA (Mouse); REC1; Cell cycle checkpoint protein RAD1; mRAD1; DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein; RAD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 280

• Applicable Applications for RAD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAD1 sirna

siRNA to inhibit RAD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 575403046
• NCBI GeneID: 19355
• NCBI Accession #: NP_001276376.1
• NCBI GenBank Nucleotide #: NM_001289447.1
• UniProt Accession #: Q9QWZ1; O70452; O88391; Q3TGU1; Q3UG66; Q9QWZ3
• Molecular Weight: 30,565 Da
• NCBI Official Full Name: cell cycle checkpoint protein RAD1 isoform 1
• UniProt Protein Name: Cell cycle checkpoint protein RAD1
• Protein Family: DNA repair protein
• UniProt Gene Name: Rad1
• UniProt Synonym Gene Names: Rec1; mRAD1
• UniProt Entry Name: RAD1_MOUSE

Uniprot Description

RAD1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. Belongs to the rad1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.11.2; Cell cycle regulation; Deoxyribonuclease; DNA repair, damage

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; chromosome; nucleus

Molecular Function: nuclease activity; hydrolase activity; exonuclease activity; exodeoxyribonuclease III activity; damaged DNA binding; 3'-5' exonuclease activity

Biological Process: meiotic recombination checkpoint; DNA damage checkpoint; DNA repair; response to DNA damage stimulus