RAC3 sirna

RAC3 siRNA (Mouse)

Cat. Num. AAA8205347

• Gene Names: Rac3; Rac1B
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAC3; RAC3 siRNA (Mouse); Ras-related C3 botulinum toxin substrate 3; p21-Rac3; RAC3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAC3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 192

• Applicable Applications for RAC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAC3 sirna

siRNA to inhibit RAC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18875380
• NCBI GeneID: 170758
• NCBI Accession #: NP_573486.1
• NCBI GenBank Nucleotide #: NM_133223.4
• UniProt Accession #: P60764; O14658
• Molecular Weight: 21,379 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 3
• NCBI Official Synonym Full Names: RAS-related C3 botulinum substrate 3
• NCBI Official Symbol: Rac3
• NCBI Official Synonym Symbols: Rac1B
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 3
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 3
• Protein Family: Rac-like GTP-binding protein
• UniProt Gene Name: Rac3
• UniProt Entry Name: RAC3_MOUSE

Uniprot Description

RAC3: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation. Highest levels in brain, also detected in heart, placenta and pancreas. Belongs to the small GTPase superfamily. Rho family.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, Rho

Cellular Component: filamentous actin; neuron projection; cytoskeleton; cell projection; growth cone; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; endomembrane system; intracellular

Molecular Function: GTPase activity; protein binding; GTP binding; nucleotide binding; calcium-dependent protein binding

Biological Process: metabolic process; homeostasis of number of cells within a tissue; small GTPase mediated signal transduction; neuromuscular process; synaptic transmission, GABAergic; positive regulation of cell adhesion mediated by integrin; regulation of cell morphogenesis; cerebral cortex GABAergic interneuron development; regulation of neuron maturation; cell projection biogenesis; neuromuscular process controlling balance; actin cytoskeleton organization and biogenesis; neurite development