RAC1 sirna

RAC1 siRNA (Mouse)

Cat. Num. AAA8217320

• Gene Names: Rac1; AL023026; D5Ertd559e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAC1; RAC1 siRNA (Mouse); Ras-related C3 botulinum toxin substrate 1; p21-Rac1; RAC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 192

• Applicable Applications for RAC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAC1 sirna

siRNA to inhibit RAC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 45592934
• NCBI GeneID: 19353
• NCBI Accession #: NP_033033.1
• NCBI GenBank Nucleotide #: NM_009007.2
• UniProt Accession #: P63001; O95501; P15154; Q9BTB4
• Molecular Weight: 21,450 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 1
• NCBI Official Synonym Full Names: RAS-related C3 botulinum substrate 1
• NCBI Official Symbol: Rac1
• NCBI Official Synonym Symbols: AL023026; D5Ertd559e
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: Rac1
• UniProt Entry Name: RAC1_MOUSE

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: G protein, monomeric, Rho; G protein, monomeric; G protein; Motility/polarity/chemotaxis

Cellular Component: extrinsic to plasma membrane; focal adhesion; cytoplasmic membrane-bound vesicle; actin filament; trans-Golgi network; cytosol; Golgi membrane; cell projection; membrane; lamellipodium; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; thioesterase binding; histone deacetylase binding; Rho GDP-dissociation inhibitor binding; nucleotide binding; protein kinase binding; Rab GTPase binding

Biological Process: axon guidance; metabolic process; cell motility involved in cell locomotion; regulation of neuron maturation; chemotaxis; regulation of cell migration; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; small GTPase mediated signal transduction; positive regulation of stress fiber formation; dendrite development; regulation of cell morphogenesis; cell adhesion; bone resorption; lamellipodium biogenesis; embryonic olfactory bulb interneuron precursor migration; intercellular junction assembly and maintenance; cell migration; Wnt receptor signaling pathway, planar cell polarity pathway; mast cell chemotaxis; dendrite morphogenesis; synaptic transmission, GABAergic; positive regulation of phosphoinositide 3-kinase activity; actin filament organization; cytoskeleton organization and biogenesis; endocytosis; engulfment of apoptotic cell; cerebral cortex GABAergic interneuron development; cell proliferation; G-protein coupled receptor protein signaling pathway; hyperosmotic response; negative regulation of interleukin-23 production; positive regulation of actin filament polymerization; homeostasis of number of cells within a tissue; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; cerebral cortex radially oriented cell migration; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication