RAB8A sirna

RAB8A siRNA (Mouse)

Cat. Num. AAA8239221

• Gene Names: Rab8a; Mel; AA409338
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB8A; RAB8A siRNA (Mouse); MEL; RAB8; Ras-related protein Rab-8A; Oncogene c-mel; RAB8A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAB8A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 207

• Applicable Applications for RAB8A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAB8A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB8A sirna

siRNA to inhibit RAB8A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38372905
• NCBI GeneID: 17274
• NCBI Accession #: NP_075615.2
• NCBI GenBank Nucleotide #: NM_023126.2
• UniProt Accession #: P55258; Q8VCF6
• Molecular Weight: 23,668 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: Rab8a
• NCBI Official Synonym Symbols: Mel; AA409338
• NCBI Protein Information: ras-related protein Rab-8A
• UniProt Protein Name: Ras-related protein Rab-8A
• Protein Family: Ras-related protein
• UniProt Gene Name: Rab8a
• UniProt Synonym Gene Names: Mel; Rab8
• UniProt Entry Name: RAB8A_MOUSE

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab; Oncoprotein; Motility/polarity/chemotaxis; G protein

Cellular Component: Golgi apparatus; centrosome; recycling endosome membrane; secretory granule membrane; nonmotile primary cilium; postsynaptic density; dendrite; cytoplasmic membrane-bound vesicle; phagocytic vesicle; cilium; nucleoplasm; trans-Golgi network transport vesicle; recycling endosome; synaptic vesicle; cell projection; membrane; cell soma; nucleolus; plasma membrane; cytoplasmic vesicle; nucleus; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; nucleotide binding; myosin V binding; protein kinase binding

Biological Process: regulation of long-term neuronal synaptic plasticity; metabolic process; protein secretion; regulation of exocytosis; regulation of protein transport; intracellular protein transport; synaptic vesicle exocytosis; protein transport; regulation of protein localization; axonogenesis; Golgi vesicle fusion to target membrane; cell projection organization and biogenesis; cellular response to insulin stimulus; transport; small GTPase mediated signal transduction; cilium biogenesis; Rab protein signal transduction; vesicle docking during exocytosis