RAB25 sirna

RAB25 siRNA (Mouse)

Cat. Num. AAA8219186

• Gene Names: Rab25; AV012978
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB25; RAB25 siRNA (Mouse); Ras-related protein Rab-25; RAB25 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAB25 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 213

• Applicable Applications for RAB25 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAB25 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB25 sirna

siRNA to inhibit RAB25 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31980838
• NCBI GeneID: 53868
• NCBI Accession #: NP_058595.2
• NCBI GenBank Nucleotide #: NM_016899.4
• UniProt Accession #: Q9WTL2; Q9D1P3
• Molecular Weight: 23,473 Da
• NCBI Official Full Name: ras-related protein Rab-25
• NCBI Official Synonym Full Names: RAB25, member RAS oncogene family
• NCBI Official Symbol: Rab25
• NCBI Official Synonym Symbols: AV012978
• NCBI Protein Information: ras-related protein Rab-25
• UniProt Protein Name: Ras-related protein Rab-25
• Protein Family: Ras-related protein
• UniProt Gene Name: Rab25
• UniProt Entry Name: RAB25_MOUSE

Uniprot Description

RAB25: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis. Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP- dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Cellular Component: recycling endosome; cell projection; membrane; plasma membrane; pseudopodium; cytoplasmic vesicle

Molecular Function: GTPase activity; protein binding; myosin V binding; nucleotide binding

Biological Process: intracellular protein transport; protein transport; exocytosis; transport; small GTPase mediated signal transduction; positive regulation of cell proliferation; Rab protein signal transduction; pseudopodium organization and biogenesis; melanosome transport