QKI sirna

QKI siRNA (Mouse)

Cat. Num. AAA8225116

• Gene Names: Qk; QkI; l17Wis1; 1500005P18; l(17)-1Wis; 1110003F05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: QKI; QKI siRNA (Mouse); QK; QK1; QKA1; Protein quaking; MqkI; qkI; QKI sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: QKI siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 319

• Applicable Applications for QKI sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse QKI gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for QKI sirna

siRNA to inhibit QKI expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226958438
• NCBI GeneID: 19317
• NCBI Accession #: NP_001152988.1
• NCBI GenBank Nucleotide #: NM_001159516.1
• UniProt Accession #: Q9QYS9; O88972; Q61110; Q78ZE4; Q78ZE5; Q8K4X9; Q8K4Y0; Q9CW34; Q9QUH4; Q9R2A8; Q9Z246
• Molecular Weight: 35,227 Da
• NCBI Official Full Name: protein quaking isoform 2
• NCBI Official Synonym Full Names: quaking
• NCBI Official Symbol: Qk
• NCBI Official Synonym Symbols: QkI; l17Wis1; 1500005P18; l(17)-1Wis; 1110003F05Rik
• NCBI Protein Information: protein quaking
• UniProt Protein Name: Protein quaking
• Protein Family: Protein quaking
• UniProt Gene Name: Qki
• UniProt Synonym Gene Names: Qk; Qk1; Qka1; MqkI; qkI
• UniProt Entry Name: QKI_MOUSE

Uniprot Description

QKI: RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. 6 isoforms of the human protein are produced by alternative splicing.

Cellular Component: cytoplasm; nucleus

Molecular Function: mRNA binding; nucleic acid binding; RNA binding; SH3 domain binding

Biological Process: myelination; regulation of translation; muscle cell differentiation; mRNA transport; transport; RNA splicing; multicellular organismal development; long-chain fatty acid biosynthetic process; axon ensheathment; mRNA processing; cell differentiation; vasculogenesis; spermatid development