PVRL3 sirna

PVRL3 siRNA (Mouse)

Cat. Num. AAA8214404

• Gene Names: Pvrl3; CD113; AA407785; AU016832; AW538082; 2610301B19Rik; 3000002N23Rik; 4921513D19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PVRL3; PVRL3 siRNA (Mouse); Poliovirus receptor-related protein 3; Nectin-3; CD antigen CD113; PVRL3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PVRL3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 549

• Applicable Applications for PVRL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PVRL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PVRL3 sirna

siRNA to inhibit PVRL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12957492
• NCBI GeneID: 58998
• NCBI Accession #: NP_067470.1
• NCBI GenBank Nucleotide #: NM_021495.4
• UniProt Accession #: Q9JLB9; Q059N7; Q9D006; Q9JLB7; Q9JLB8
• Molecular Weight: 47,262 Da
• NCBI Official Full Name: nectin-3 isoform alpha
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: Pvrl3
• NCBI Official Synonym Symbols: CD113; AA407785; AU016832; AW538082; 2610301B19Rik; 3000002N23Rik; 4921513D19Rik
• NCBI Protein Information: nectin-3
• UniProt Protein Name: Nectin-3
• UniProt Gene Name: Pvrl3
• UniProt Entry Name: PVRL3_MOUSE

Uniprot Description

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; membrane; plasma membrane; integral to membrane; synapse; intercellular junction; cell junction

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; cell adhesion; homophilic cell adhesion; retina morphogenesis in camera-type eye