PTPRU sirna

PTPRU siRNA (Mouse)

Cat. Num. AAA8237685

• Gene Names: Ptpru; PTP; Pcp2; Ptpf; Ftp-1; PCP-2; Ptprl; R-PTP-U; PTPlambda; R-PTP-psi; PTP-lambda; RPTPlambda
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPRU; PTPRU siRNA (Mouse); PCP2; PTPF; Receptor-type tyrosine-protein phosphatase U; R-PTP-U; Ftp-1; Pancreatic carcinoma phosphatase 2; PCP-2; Protein-tyrosine phosphatase-lamda; PTP-lambda; PTPlambda; Receptor-type protein-tyrosine phosphatase psi; R-PTP-psi; PTPRU sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTPRU siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,436

• Applicable Applications for PTPRU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTPRU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPRU sirna

siRNA to inhibit PTPRU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 133778922
• NCBI GeneID: 19273
• NCBI Accession #: NP_001076588.1
• NCBI GenBank Nucleotide #: NM_001083119.2
• UniProt Accession #: B1AUH1; O35564; P70125; Q3V312; Q66JV9
• Molecular Weight: 161,158 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase U isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, U
• NCBI Official Symbol: Ptpru
• NCBI Official Synonym Symbols: PTP; Pcp2; Ptpf; Ftp-1; PCP-2; Ptprl; R-PTP-U; PTPlambda; R-PTP-psi; PTP-lambda; RPTPlambda
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase U
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase U
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: Ptpru
• UniProt Synonym Gene Names: Pcp2; Ptpf; R-PTP-U; PCP-2; PTP-lambda; PTPlambda; R-PTP-psi
• UniProt Entry Name: PTPRU_MOUSE

Uniprot Description

PTPRU: Tyrosine-protein phosphatase which dephosphorylates CTNNB1. Regulates CTNNB1 function both in cell adhesion and signaling. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. May play a role in megakaryocytopoiesis. Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.1.3.48; Receptor protein phosphatase, tyrosine

Cellular Component: membrane; integral to membrane; plasma membrane; intercellular junction; cell junction

Molecular Function: hydrolase activity; phosphoric monoester hydrolase activity; beta-catenin binding; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of cell proliferation; cell-cell adhesion; dephosphorylation; homotypic cell-cell adhesion; Wnt receptor signaling pathway through beta-catenin; protein amino acid dephosphorylation; cell adhesion; cell differentiation; negative regulation of cell migration