PTPRJ sirna

PTPRJ siRNA (Mouse)

Cat. Num. AAA8221459

• Gene Names: Ptprj; BET; Byp; Scc1; CD148; DEP-1; Ptpb2; RPTPJ; Scc-1; AI450271; PTPbeta2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPRJ; PTPRJ siRNA (Mouse); BYP; SCC1; Receptor-type tyrosine-protein phosphatase eta; Protein-tyrosine phosphatase eta; R-PTP-eta; HPTP beta-like tyrosine phosphatase; Protein-tyrosine phosphatase receptor type J; R-PTP-J; Susceptibility to colon cancer 1; CD antigen CD148; PTPRJ sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTPRJ siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1350

• Applicable Applications for PTPRJ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTPRJ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPRJ sirna

siRNA to inhibit PTPRJ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 208609939
• NCBI GeneID: 19271
• NCBI Accession #: NP_033008.3
• NCBI GenBank Nucleotide #: NM_008982.5
• UniProt Accession #: Q64455; Q3UH64; Q3UHL5; Q541R5; Q8CIW9; Q8K3Q2
• Molecular Weight: 136,769 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase eta isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, J
• NCBI Official Symbol: Ptprj
• NCBI Official Synonym Symbols: BET; Byp; Scc1; CD148; DEP-1; Ptpb2; RPTPJ; Scc-1; AI450271; PTPbeta2
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase eta
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase eta
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: Ptprj
• UniProt Synonym Gene Names: Byp; Scc1; Protein-tyrosine phosphatase eta; R-PTP-eta; R-PTP-J
• UniProt Entry Name: PTPRJ_MOUSE

Uniprot Description

PTPRJ: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis; EC 3.1.3.48

Cellular Component: nucleoplasm; cell surface; cell projection; membrane; integral to membrane; plasma membrane; immunological synapse; intercellular junction; cell junction

Molecular Function: hydrolase activity; gamma-catenin binding; phosphoric monoester hydrolase activity; platelet-derived growth factor receptor binding; beta-catenin binding; mitogen-activated protein kinase binding; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; regulation of cell adhesion; negative regulation of MAP kinase activity; positive regulation of cell adhesion; negative regulation of protein kinase B signaling cascade; heart development; negative regulation of T cell receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; dephosphorylation; positive regulation of focal adhesion formation; positive chemotaxis; negative regulation of vascular permeability; oligodendrocyte differentiation; negative regulation of cell growth; positive regulation of cell-matrix adhesion; vasculogenesis; blood coagulation; negative regulation of cell migration