PTK2B sirna

PTK2B siRNA (Mouse)

Cat. Num. AAA8229838

• Gene Names: Ptk2b; CAKB; FAK2; PYK2; CADTK; FADK2; Raftk; CAKbeta; E430023O05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTK2B; PTK2B siRNA (Mouse); FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase; PTK2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTK2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1009

• Applicable Applications for PTK2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTK2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTK2B sirna

siRNA to inhibit PTK2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 241982789
• NCBI GeneID: 19229
• NCBI Accession #: NP_001155838.1
• NCBI GenBank Nucleotide #: NM_001162366.1
• UniProt Accession #: Q9QVP9; B2RQ16; G3X8V1
• Molecular Weight: 115,794 Da
• NCBI Official Full Name: protein-tyrosine kinase 2-beta isoform 1
• NCBI Official Synonym Full Names: PTK2 protein tyrosine kinase 2 beta
• NCBI Official Symbol: Ptk2b
• NCBI Official Synonym Symbols: CAKB; FAK2; PYK2; CADTK; FADK2; Raftk; CAKbeta; E430023O05Rik
• NCBI Protein Information: protein-tyrosine kinase 2-beta
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: Ptk2b
• UniProt Synonym Gene Names: Fak2; Pyk2; Raftk; CADTK; CAK-beta; CAKB; FADK 2; RAFTK
• UniProt Entry Name: FAK2_MOUSE

Uniprot Description

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; Nuclear receptor co-regulator; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Fak family

Cellular Component: focal adhesion; postsynaptic density; dendrite; cell cortex; cytosol; N-methyl-D-aspartate selective glutamate receptor complex; lipid raft; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; cell projection; cytoskeleton; membrane; cell soma; axon; lamellipodium; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cell junction

Molecular Function: calmodulin-dependent protein kinase activity; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; protein kinase activity; transferase activity; signal transducer activity; protein binding; 3-phosphoinositide-dependent protein kinase binding; protein-tyrosine kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; transferase activity, transferring phosphorus-containing groups; protein complex binding; kinase activity; ATP binding; receptor binding

Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; negative regulation of bone mineralization; regulation of cell shape; regulation of inositol trisphosphate biosynthetic process; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; negative regulation of ossification; negative regulation of neuron apoptosis; cell adhesion; neurite development; bone resorption; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; actin filament organization; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; ionotropic glutamate receptor signaling pathway; negative regulation of apoptosis; activation of JAK protein; peptidyl-tyrosine phosphorylation; blood vessel endothelial cell migration; positive regulation of translation; negative regulation of potassium ion transport; regulation of calcium-mediated signaling; positive regulation of JNK cascade; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; angiogenesis; positive regulation of cell-matrix adhesion; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; immune system process; MAPKKK cascade; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; positive regulation of actin filament polymerization; response to hydrogen peroxide; positive regulation of protein kinase activity; stress fiber formation; innate immune response; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; phosphorylation; positive regulation of cell migration