PRDM16 sirna

PRDM16 siRNA (Mouse)

Cat. Num. AAA827801

• Gene Names: Prdm16; csp1; mel1; 5730557K01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM16; PRDM16 siRNA (Mouse); KIAA1675; MEL1; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1; MDS1/EVI1-like gene 1; PRDM16 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PRDM16 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,276

• Applicable Applications for PRDM16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PRDM16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM16 sirna

siRNA to inhibit PRDM16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 597517895
• NCBI GeneID: 70673
• NCBI Accession #: NP_001277955.1
• NCBI GenBank Nucleotide #: NM_001291026.1
• UniProt Accession #: A2A935; Q69ZD6; Q6PB79; Q7TPF4
• Molecular Weight: 140,945 Da
• NCBI Official Full Name: PR domain zinc finger protein 16 isoform 3
• NCBI Official Synonym Full Names: PR domain containing 16
• NCBI Official Symbol: Prdm16
• NCBI Official Synonym Symbols: csp1; mel1; 5730557K01Rik
• NCBI Protein Information: PR domain zinc finger protein 16
• UniProt Protein Name: PR domain zinc finger protein 16
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: Prdm16
• UniProt Synonym Gene Names: MDS1/EVI1-like gene 1
• UniProt Entry Name: PRD16_MOUSE

Uniprot Description

PRDM16: binds DNA and functions as a transcriptional regulator. A repressor of TGF-beta signaling. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. Four human isoforms are produced by alternative promoter usage and alternative splicing

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: protein binding; transcription activator binding; DNA binding; nucleic acid binding; sequence-specific DNA binding; metal ion binding; transcription coactivator activity; SMAD binding

Biological Process: tongue development; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; stem cell maintenance; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of granulocyte differentiation; regulation of cellular respiration; neurogenesis; regulation of transcription, DNA-dependent; white fat cell differentiation; brown fat cell differentiation; negative regulation of transcription, DNA-dependent; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway