PPP2R2A sirna

PPP2R2A siRNA (Mouse)

Cat. Num. AAA8220734

• Gene Names: Ppp2r2a; mKIAA1541; 2410004D02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP2R2A; PPP2R2A siRNA (Mouse); Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha; PPP2R2A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PPP2R2A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 367

• Applicable Applications for PPP2R2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PPP2R2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP2R2A sirna

siRNA to inhibit PPP2R2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 327180707
• NCBI GeneID: 71978
• NCBI Accession #: NP_001192117.1
• NCBI GenBank Nucleotide #: NM_001205188.1
• UniProt Accession #: Q6P1F6
• Molecular Weight: 51,692 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit B, alpha
• NCBI Official Symbol: Ppp2r2a
• NCBI Official Synonym Symbols: mKIAA1541; 2410004D02Rik
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: Ppp2r2a
• UniProt Entry Name: 2ABA_MOUSE

Uniprot Description

PPP2R2A: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Expressed in all tissues examined. Belongs to the phosphatase 2A regulatory subunit B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Cellular Component: protein phosphatase type 2A complex

Molecular Function: protein phosphatase 2A binding; protein complex binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; tau protein binding

Biological Process: response to morphine; protein amino acid dephosphorylation; signal transduction