PPAP2A sirna

PPAP2A siRNA (Mouse)

Cat. Num. AAA8240946

• Gene Names: Ppap2a; LPP1; mPAP; Hic53; LPP-1; PAP2a; Hpic53; PAP-2a; PAP2-alpha
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPAP2A; PPAP2A siRNA (Mouse); HPIC53; LPP1; Lipid phosphate phosphohydrolase 1; 35 kDa PAP; mPAP; Hydrogen peroxide-inducible protein 53; Hic53; PAP2-alpha; Phosphatidate phosphohydrolase type 2a; Phosphatidic acid phosphatase 2a; PAP-2a; PAP2a; PPAP2A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PPAP2A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 284

• Applicable Applications for PPAP2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PPAP2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPAP2A sirna

siRNA to inhibit PPAP2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 45592928
• NCBI GeneID: 19012
• NCBI Accession #: NP_032273.1
• NCBI GenBank Nucleotide #: NM_008247.3
• UniProt Accession #: Q61469; Q61690; Q6GT30; Q8BPB8
• Molecular Weight: 31,996 Da
• NCBI Official Full Name: lipid phosphate phosphohydrolase 1 isoform 1
• NCBI Official Synonym Full Names: phosphatidic acid phosphatase type 2A
• NCBI Official Symbol: Ppap2a
• NCBI Official Synonym Symbols: LPP1; mPAP; Hic53; LPP-1; PAP2a; Hpic53; PAP-2a; PAP2-alpha
• NCBI Protein Information: lipid phosphate phosphohydrolase 1
• UniProt Protein Name: Lipid phosphate phosphohydrolase 1
• UniProt Gene Name: Ppap2a
• UniProt Synonym Gene Names: Hpic53; Lpp1; mPAP; Hic53; PAP-2a; PAP2a
• UniProt Entry Name: LPP1_MOUSE

Uniprot Description

PPAP2A: Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). Pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. Major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. May control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. It's down-regulation may contribute to the development of colon adenocarcinoma. Belongs to the PA-phosphatase related phosphoesterase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Lipid Metabolism - glycerolipid; Lipid Metabolism - sphingolipid; EC 3.1.3.4; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Phosphatase (non-protein); Lipid Metabolism - ether lipid

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: hydrolase activity; phosphatidate phosphatase activity; catalytic activity

Biological Process: dephosphorylation; diacylglycerol biosynthetic process; lipid metabolic process; sphingosine metabolic process; signal transduction; ceramide metabolic process; protein amino acid dephosphorylation