PGM3 sirna

PGM3 siRNA (Mouse)

Cat. Num. AAA8201867

• Gene Names: Pgm3; Agm1; PAGM; Pgm-3; C77933; BB187688; 2810473H05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PGM3; PGM3 siRNA (Mouse); AGM1; PGM-3; Phosphoacetylglucosamine mutase; PAGM; Acetylglucosamine phosphomutase; N-acetylglucosamine-phosphate mutase; Phosphoglucomutase-3; PGM 3; PGM3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PGM3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 501

• Applicable Applications for PGM3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PGM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PGM3 sirna

siRNA to inhibit PGM3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255522941
• NCBI GeneID: 109785
• NCBI Accession #: NP_001157218.1
• NCBI GenBank Nucleotide #: NM_001163746.1
• UniProt Accession #: Q9CYR6; Q543F9; B2RS40
• Molecular Weight: 59,453 Da
• NCBI Official Full Name: phosphoacetylglucosamine mutase isoform 2
• NCBI Official Synonym Full Names: phosphoglucomutase 3
• NCBI Official Symbol: Pgm3
• NCBI Official Synonym Symbols: Agm1; PAGM; Pgm-3; C77933; BB187688; 2810473H05Rik
• NCBI Protein Information: phosphoacetylglucosamine mutase
• UniProt Protein Name: Phosphoacetylglucosamine mutase
• UniProt Gene Name: Pgm3
• UniProt Synonym Gene Names: PGM 3
• UniProt Entry Name: AGM1_MOUSE

Uniprot Description

PGM3: Interconverts GlcNAc-6-P and GlcNAc-1-P. Belongs to the phosphohexose mutase family.

Protein type: EC 5.4.2.3; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase

Cellular Component: cytosol

Molecular Function: phosphoglucomutase activity; intramolecular transferase activity, phosphotransferases; metal ion binding; magnesium ion binding; phosphoacetylglucosamine mutase activity; isomerase activity

Biological Process: protein amino acid O-linked glycosylation; embryonic development; carbohydrate metabolic process; protein amino acid N-linked glycosylation; UDP-N-acetylglucosamine biosynthetic process; glucose 1-phosphate metabolic process; spermatogenesis; hemopoiesis