PFKM sirna

PFKM siRNA (Mouse)

Cat. Num. AAA8236093

• Gene Names: Pfkm; Pfk4; Pfka; Pfkx; PFK-A; PFK-M; Pfk-4; ATP-PFK; AI131669
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PFKM; PFKM siRNA (Mouse); PFK-M; PFKA; 6-phosphofructokinase. muscle type; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase; PFKM sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PFKM siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 931

• Applicable Applications for PFKM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PFKM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PFKM sirna

siRNA to inhibit PFKM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 254553346
• NCBI GeneID: 18642
• NCBI Accession #: NP_001156959.1
• NCBI GenBank Nucleotide #: NM_001163487.1
• UniProt Accession #: P47857; O35513; Q543L1; Q9JK94; C8CMN5; C8CMN6; C8CMN7
• Molecular Weight: 102,384 Da
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, muscle type
• NCBI Official Synonym Full Names: phosphofructokinase, muscle
• NCBI Official Symbol: Pfkm
• NCBI Official Synonym Symbols: Pfk4; Pfka; Pfkx; PFK-A; PFK-M; Pfk-4; ATP-PFK; AI131669
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, muscle type
• UniProt Protein Name: ATP-dependent 6-phosphofructokinase, muscle type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: Pfkm
• UniProt Synonym Gene Names: Pfk-m; Pfka; ATP-PFK; PFK-M
• UniProt Entry Name: PFKAM_MOUSE

Uniprot Description

PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - fructose and mannose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway

Cellular Component: cell projection; 6-phosphofructokinase complex; cytoplasm; apical plasma membrane; cytosol; cilium

Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; metal ion binding; nucleotide binding; transferase activity; protein binding; kinase activity; carbohydrate binding; kinase binding; phosphofructokinase activity; catalytic activity; 6-phosphofructokinase activity; ATP binding; AMP binding

Biological Process: muscle maintenance; fructose 1,6-bisphosphate metabolic process; glycolysis; metabolic process; carbohydrate phosphorylation; positive regulation of insulin secretion; glucose homeostasis; fructose 6-phosphate metabolic process; phosphorylation; protein oligomerization; protein homotetramerization