PEX6 sirna

PEX6 siRNA (Mouse)

Cat. Num. AAA8205862

• Gene Names: Pex6; AI132582; mKIAA4177; D130055I09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PEX6; PEX6 siRNA (Mouse); Peroxisome assembly factor 2; PAF-2; Peroxin-6; Peroxisomal biogenesis factor 6; Peroxisomal-type ATPase 1; PEX6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PEX6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 981

• Applicable Applications for PEX6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PEX6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PEX6 sirna

siRNA to inhibit PEX6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21703962
• NCBI GeneID: 224824
• NCBI Accession #: NP_663463.1
• NCBI GenBank Nucleotide #: NM_145488.1
• UniProt Accession #: Q99LC9; Q6YNQ9
• Molecular Weight: 104,549 Da
• NCBI Official Full Name: peroxisome assembly factor 2
• NCBI Official Synonym Full Names: peroxisomal biogenesis factor 6
• NCBI Official Symbol: Pex6
• NCBI Official Synonym Symbols: AI132582; mKIAA4177; D130055I09Rik
• NCBI Protein Information: peroxisome assembly factor 2
• UniProt Protein Name: Peroxisome assembly factor 2
• Protein Family: Peroxisomal ATPase
• UniProt Gene Name: Pex6
• UniProt Synonym Gene Names: PAF-2
• UniProt Entry Name: PEX6_MOUSE

Uniprot Description

PEX6: Involved in peroxisome biosynthesis. Required for stability of the PTS1 receptor. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes. Defects in PEX6 are the cause of peroxisome biogenesis disorder complementation group 4 (PBD-CG4); also known as PBD-CGC. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Defects in PEX6 are a cause of Zellweger syndrome (ZWS). ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. Belongs to the AAA ATPase family.

Protein type: Membrane protein, peripheral

Cellular Component: peroxisomal membrane; membrane; cytoplasm; peroxisome; cytosol

Molecular Function: protein C-terminus binding; protein binding; ATPase activity; nucleotide binding; protein complex binding; ATPase activity, coupled; ATP binding

Biological Process: protein stabilization; protein targeting to peroxisome; metabolic process; peroxisome organization and biogenesis; protein import into peroxisome matrix, translocation