PDK1 sirna

PDK1 siRNA (Mouse)

Cat. Num. AAA8230596

• Gene Names: Pdk1; B830012B01; D530020C15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDK1; PDK1 siRNA (Mouse); [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 1 mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 434

• Applicable Applications for PDK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDK1 sirna

siRNA to inhibit PDK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110625975
• NCBI GeneID: 228026
• NCBI Accession #: NP_766253.2
• NCBI GenBank Nucleotide #: NM_172665.5
• UniProt Accession #: Q8BFP9; Q3U5E5
• Molecular Weight: 48,995 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 1
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isoenzyme 1
• NCBI Official Symbol: Pdk1
• NCBI Official Synonym Symbols: B830012B01; D530020C15Rik
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 1
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: Pdk1
• UniProt Synonym Gene Names: PDH kinase 1
• UniProt Entry Name: PDK1_MOUSE

Uniprot Description

PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Kinase, protein; EC 2.7.11.2; Mitochondrial; Protein kinase, atypical; ATYPICAL group; PDHK family

Cellular Component: mitochondrion; plasma membrane; pyruvate dehydrogenase complex; intracellular; mitochondrial pyruvate dehydrogenase complex

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; protein complex binding; nucleotide binding; kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-serine phosphorylation; cell surface receptor linked signal transduction; carbohydrate metabolic process; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; phosphorylation; protein amino acid phosphorylation; induction of apoptosis by oxidative stress